Compartmental cross-talk in the regulation of light harvesting complex transcription under short-term light and temperature stress in <i>Chlamydomonas reinhardtii</i>
Bibliographic record
Abstract
Short-term light stress in Chlamydomonas leads to transient changes in light harvesting complex (LHC) transcription. This requires cross-talk between the plastid and nucleus to coordinate chloroplast function with nuclear transcription. None of the components of this signalling pathway have been identified although several sensor candidates have been proposed. To examine the regulation of nuclear photosynthetic gene expression, we constructed an LHC::Arylsulphatase (ARS) reporter system in Chlamydomonas reinhardtii allowing us to examine short-term regulatory changes on a fine scale. We modulated plastoquinone (PQ) redox state via photosynthetic inhibitors, changes in light and (or) temperature and found no evidence that either the PQ pool or Q A redox state were directly involved in short-term retrograde signalling. Shifts in light level and (or) temperature indicated that LHC transcriptional activity is tightly coordinated to photosynthetic production. Transient switching between photoautotrophic and mixotrophic growth, plus the use of mitochondrial inhibitors indicated that nuclear photosynthetic gene expression is coupled to mitochondrial activity. These short-term effects on LHC transcription demonstrate an interdependence of photosynthetic production and mitochondrial activity, suggesting Chlamydomonas is able to respond to environmental changes by monitoring metabolite pools between the chloroplast and mitochondria and not in the chloroplast directly.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".