Compartmental cross-talk in the regulation of light harvesting complex transcription under short-term light and temperature stress in <i>Chlamydomonas reinhardtii</i>
Bibliographic record
Abstract
Short-term light stress in Chlamydomonas leads to transient changes in light harvesting complex (LHC) transcription. This requires cross-talk between the plastid and nucleus to coordinate chloroplast function with nuclear transcription. None of the components of this signalling pathway have been identified although several sensor candidates have been proposed. To examine the regulation of nuclear photosynthetic gene expression, we constructed an LHC::Arylsulphatase (ARS) reporter system in Chlamydomonas reinhardtii allowing us to examine short-term regulatory changes on a fine scale. We modulated plastoquinone (PQ) redox state via photosynthetic inhibitors, changes in light and (or) temperature and found no evidence that either the PQ pool or QA redox state were directly involved in short-term retrograde signalling. Shifts in light level and (or) temperature indicated that LHC transcriptional activity is tightly coordinated to photosynthetic production. Transient switching between photoautotrophic and mixotrophic growth, plus the use of mitochondrial inhibitors indicated that nuclear photosynthetic gene expression is coupled to mitochondrial activity. These short-term effects on LHC transcription demonstrate an interdependence of photosynthetic production and mitochondrial activity, suggesting Chlamydomonas is able to respond to environmental changes by monitoring metabolite pools between the chloroplast and mitochondria and not in the chloroplast directly.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".