Electrochemical Studies of the Effect of Temperature on the Adsorption of Yeast Alcohol Dehydrogenase at Pt
Bibliographic record
Abstract
The interfacial behavior of yeast alcohol dehydrogenase (YADH) without and with the coenzyme nicotinamide adenine dinucleotide (NADH−YADH) at a Pt surface was studied over the temperature range 273−353 K in a phosphate buffer solution of pH 7.0, using cyclic voltammetry and electrochemical impedance spectroscopy. It was shown that the surface charge density and corresponding polarization resistance, resulting from protein adsorption and its oxidation, respectively, are directly proportional to the amount of adsorbed protein (surface concentration), indicating that adsorption at anodic potentials is accompanied by the transfer of charge, that is, chemisorption through carboxylate groups on the protein. The adsorption process for both proteins was described with the Langmuir adsorption isotherm, which revealed very high affinity of the proteins toward adsorption onto a Pt surface. From the calculated Gibbs energies of adsorption, it was concluded that both proteins strongly adsorb onto the Pt surface via chemisorption. The adsorption process for YADH was found to be exothermic. However, the adsorption of NADH−YADH resulted in an endothermic adsorption process as a result of the presence of the coenzyme, nicotinamide adenine dinucleotide (NAD + /NADH), in NADH−YADH which stabilizes the protein against denaturation. However, the adsorption of both proteins was found to be an entropically governed process, suggesting structural unfolding of the proteins at the electrode surface. The maximum surface concentration values indicate that there is no significant difference in the amount of adsorbed proteins between YADH and NADH−YADH in the whole temperature range investigated.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".