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Record W2074054887 · doi:10.1055/s-2009-1216450

Using DNA Barcodes to Identify Rosaceae

2009· article· en· W2074054887 on OpenAlexfundno aff
XH Pang, SL Chen

Bibliographic record

VenuePlanta Medica · 2009
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenomics and Phylogenetic Studies
Canadian institutionsnot available
FundersNational Institute on Drug AbuseAgricultural Research ServiceUniversity of British ColumbiaUniversity of Illinois at Urbana-ChampaignChinese Academy of SciencesKurukshetra UniversityUniversity of Illinois at ChicagoNational Oceanic and Atmospheric AdministrationU.S. Food and Drug AdministrationNational Science Foundation of Sri LankaUniversity of ColomboU.S. Department of AgricultureWestern Carolina UniversityInternational Science CouncilNational Center for Complementary and Alternative MedicineChina Academy of Traditional Chinese MedicineNational Institutes of HealthNational Center for Research ResourcesNational Institute of Allergy and Infectious DiseasesTürkiye Bilimsel ve Teknolojik Araştırma KurumuHong Kong Polytechnic UniversityNational Science FoundationDeutsche KrebshilfeNational Natural Science Foundation of ChinaTata TrustsUniversity Grants CommissionWake Forest University
KeywordsDNA barcodingInternal transcribed spacerBiologyRibosomal DNAAmpliconrpoBRibosomal RNARosaceaeDNA sequencingEvolutionary biologyElectropherogramComputational biologyGeneticsDNABotanyGenePhylogeneticsPolymerase chain reaction16S ribosomal RNA

Abstract

fetched live from OpenAlex

DNA barcoding has recently been proposed as a technique that employs a short, standardized gene region to identify species. DNA barcoding is well established in animals because of a widely appropriate sequence for them, the cytochrome coxidase 1 [1], but there is not any universally accepted barcode for plants till now. Therefore, the primary task for barcoding plants is to find more useful barcodes that can identify as many species as possible. Medicinal plants have been used as traditional Chinese drugs for treating diseases, some of them are similar in morphology, and are often misidentified by chemical fingerprints. Rosaceae includes many medicinal plants with similar morphology and are usually hard to be identified. Here, we chose five potential barcodes, Universal Plastid Amplicon (matK, rpoB, rpoC1, rbcL) and the nuclear ribosomal DNA (rDNA) internal transcribed spacer (ITS), to identify species from different genera in Rosaceae. The results suggest that the nuclear ribosomal DNA (rDNA) internal transcribed spacer (ITS) is a candidate to discriminate all of plant species in Rosaceae. Acknowledgements : We thank all my teachers and classmates in our laboratory very much for their help. References: [1] Kress WJ, et al. (2005) PNAS, 102: 8369–8374.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.003
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.005
Threshold uncertainty score0.017

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.003
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0020.001
Science and technology studies0.0010.001
Scholarly communication0.0010.001
Open science0.0010.001
Research integrity0.0020.002
Insufficient payload (model declined to judge)0.0050.005

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.027
GPT teacher head0.307
Teacher spread0.281 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations5
Published2009
Admission routes1
Has abstractyes

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