A membrane-bound glycerol-3-phosphate acyltransferase from<i>Thalassiosira pseudonana</i>regulates acyl composition of glycerolipidsThis paper is one of a selection of papers published in a Special Issue from the National Research Council of Canada – Plant Biotechnology Institute.
Bibliographic record
Abstract
To what extent fatty acyltransferases control fatty acid composition in glycerolipids is largely unknown. To gain some insight into this process, we characterized a membrane-bound glycerol-3-phosphate acyltransferase (GPAT), designated TpGPAT, from the marine diatom Thalassiosira pseudonana Hasle & Heim. The glycerolipids from T. pseudonana consist predominantly of 16:0, 16:1ω7, and 20:5ω3 fatty acids. Heterologous expression of TpGPAT in a yeast GPAT-deficient mutant (gat1) demonstrated that TpGPAT could effectively use glycerol-3-phosphate, but not dihydroxyacetone phosphate, as fatty acyl acceptor. This enzyme highly preferred 16:0 in an in vitro enzyme assay, and discriminated against the monounsaturated 16-carbon fatty acid. Accordingly, expression of TpGPAT in gat1 resulted in approximately 18% and 12% increases of 16:0 in triacylglycerols and phospholipids, respectively. The unsaturated fatty acids, 16:1 and 18:1, on the other hand, were reduced by 15% and 21% in these two lipid species. Collectively, the results demonstrate that TpGPAT possesses a high substrate preference for 16:0-CoA and it exerts a large effect on the fatty-acid composition of glycerolipids.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".