Catecholamine Neurons of the Central Nervous System in Organotypic Culture
Bibliographic record
Abstract
A standardized dissection has been designed to produce explants from the locus coeruleus of the newborn mouse brain. This area, termed the peduncular region, may be explanted with or without cerebellar neurons. Organotypic cultures with peduncular cells contain a cluster of 20-50 (or more) closely-packed large neurons, characterized by intracellular refractile granules in the living state. When treated with the glyoxylic acid method, somata of the granule-containing neurons exhibited catecholamine fluorescence, and a profuse plexus of varicose fluorescent fibers permeated the explant and its outgrowth. Intracellular staining of these cells with horseradish peroxidase demonstrated bipolar and multipolar neurons (25-30 micrometers), with stout dendrites and varicose axons. Granule-containing neurons stained for acetylcholinesterase. Electron microscopically, large dense granules were seen, which appear to correspond to the granules seen in living neurons. Cultures with these cells also have a high concentration of catecholamines in the medium. It is concluded that the granule-bearing neurons of the living culture are in fact neurons of the locus coeruleus.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.002 | 0.001 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.003 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".