Cloning, characterization and expression of two glutathione <i>S</i>‐transferase cDNAs in the spruce budworm, <i>Choristoneura fumiferana</i>
Bibliographic record
Abstract
Two Choristoneura fumiferana glutathione S-transferase cDNAs (CfGSTs4 and CfGSTd5) were cloned from a cDNA library constructed using mRNA from the midgut cell line, CF-203. These cDNAs encoded two structurally different proteins with a predicted molecular mass of 23 and 24 kDa, respectively. Amino acid sequence analysis indicates that CfGSTs4 and CfGSTd5 contained Sigma and Delta GST domain, respectively. CfGSTs4 cDNA was expressed as a recombinant protein with the same molecular mass as predicted. Semi-quantitative reverse-transcription PCR analyses indicated that both of these genes were expressed in the epidermis, fat body, and midgut of the 6th instar larvae, as well as CF-203 cells. CfGSTs4 was highly and almost constantly expressed in all tissues during the 6th instar stage. There were higher levels of CfGSTs4 protein in the midgut and fat body than in the epidermis. CfGSTd5 was expressed in the fat body when the insects underwent pupal molting and was constantly expressed in the epidermis and midgut during 6th instar development. CfGSTs4 expression was not affected by ecdysone agonist tebufenozide (RH5992), whereas CfGSTd5 expression was slightly suppressed by the compound.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".