Polerovirus ORF0 genes induce a host-specific response resembling viral infection
Bibliographic record
Abstract
Constitutive expression of the ORF0 (open reading frame 0) gene product from the Potato leafroll virus (PLRV) in transgenic host potato plants is known to produce viral-disease-like symptoms. To determine whether these symptoms were the result of a host-specific response to transgene expression, PLRV ORF0 and the orthologous-ORF0 genes of the closely related poleroviruses Beet western yellows virus (BWYV) and Cucurbit aphid-borne yellows virus (CABYV) were stably transformed into Solanum tuberosum and Nicotiana benthamiana plants. Only potato plants expressing the PLRV ORF0 displayed symptoms typical of virus infection, indicating a host-specific response of potato to the ORF0 gene product of the naturally infecting virus PLRV. Nicotiana benthamiana plants transgenic for PLRV ORF0 were phenotypically indistinguishable from pBIN19-transgenic and nontransformed plants, and symptom development was not obtained with potato or tobacco plants transgenic for ORF0 from BWYV or CABYV. Symptom development in potato plants transgenic for PLRV ORF0 coincided with an abnormal sugar metabolism resulting in an unusually high accumulation of starch in mesophyll cells. Tissue-specific expression of ORF0 in potato tubers resulted in abnormal tuber formation, reduced dormancy, and increased starch accumulation.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".