Regulation of castasterone level in primary roots of maize, <i>Zea mays</i>
Bibliographic record
Abstract
Gas chromatography–mass spectrometry analysis revealed that primary roots of maize contain 28‐norcastasterone (28‐norCS) and its biosynthetic precursors, cholesterol, and cholestanol, which suggests that the C27‐brassinosteroid (C27‐BR) biosynthetic pathway to generate 28‐norCS is operative in the roots. A cell‐free enzyme solution prepared from maize roots successfully mediated C24‐methylation of 28‐norCS to produce castasterone (CS) with the aid of S‐adenosyl‐l‐methionine, which indicates that CS can be generated through C27‐BR biosynthesis, as well as C28‐BR biosynthesis, in maize roots. Enzymatic conversion study using the cell‐free enzyme solution demonstrated that CS is converted into 26‐norCS in the enzyme solution. Exogenously applied 28‐norCS and 26‐norCS showed less activity than CS in the activation of gravitropic curvature and inhibition of root elongation. Taken together, a steady‐state level of CS, the active BR in maize roots, seems to be strictly controlled by complicated processes such as C28‐ and C27‐BR biosynthesis and biodegradation by C26‐demethylation to exert its biological activity.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".