Determination of the Complete Amino Acid Sequence for the Coat Protein of Brome Mosaic Virus by Time-of-Flight Mass Spectrometry

Time-of-flight mass spectrometry (TOFMS) has been applied to determine the complete coat protein amino acid sequences of a number of distinct brome mosaic virus (BMV) isolates. Ionization was carried out by both electrospray ionization and matrix-assisted laser desorption/ionization (MALDI). After determining overall coat protein masses, the proteins were digested with trypsin or Lys-C proteinases, and the digestion products were analyzed a mass of the coat protein was to the amino acid was to the of the the amino acid were the were coat protein sequences were with the by a the of a virus the the complete of coat protein sequences to the sequences and the Time-of-flight mass spectrometry (TOFMS) has been applied to determine the complete coat protein amino acid sequences of a number of distinct brome mosaic virus (BMV) isolates. Ionization was carried out by both electrospray ionization and matrix-assisted laser desorption/ionization (MALDI). After determining overall coat protein masses, the proteins were digested with trypsin or Lys-C proteinases, and the digestion products were analyzed a mass of the coat protein was to the amino acid was to the of the the amino acid were the were coat protein sequences were with the by a the of a virus the the complete of coat protein sequences to the sequences and the electrospray ionization brome mosaic virus coat protein matrix-assisted laser desorption/ionization mass spectrometry the a virus the mass and the amino acid of the coat been by a the and of proteins mass by amino acid sequences the been acid sequences of to by the to a complete of the coat protein the of electrospray ionization and matrix-assisted laser desorption/ionization of mass mass spectrometry a and virus coat protein sequences of the and the of of the and and of the and and of the and and of the and of the mass of the coat protein carried out with of a a of to coat proteins of or of the virus of the and by the protein with and the digestion products by the of the protein sequences with acid and the the amino acid mass spectrometry a by mass and to with the of a by a mass the of and the of amino acid sequences protein applied to a number of of the of has and and to the by and coat protein amino acid the with and to and of to and of to has the of and to the of of brome mosaic virus (BMV) of the and the of the coat protein of virus by mass spectrometry (TOFMS) of the and and the complete amino acid sequences of of and of the and and of the and and of the and to isolates. of to the by or of applied to virus a of of brome mosaic virus virus the of the and of and the and was of the to the of and has been and and and to of of the and of the protein the coat proteins of the the of the coat protein of the the acid of the and the of a number of the coat proteins carried out a of and digestion of the to the the of the coat protein was acid was the mass and a mass the the protein was to of and the to of the the of of amino of the and were the and and the the of the Lys-C were carried and the of the were by mass and the with the to the of the and and of the and and of and the were a the and mass the mass of the with the a was by the of a complete of with a complete of of the the the the of with of the coat protein of the the isolates. to the to determine the complete coat protein sequences of the of a Lys-C of the coat were by the mass and the by the of of the of a Lys-C of the coat was carried out a mass with with was the of a Lys-C of the coat protein by and of the of the a mass of the by the of the by the acid of the of the the of coat protein mass and the of with the with the of the of both and and and the with the mass of and the to with the overall mass a of and by the of and with a of the acid to with the the mass and and the to and of the and and of the and a with of the has the of the the the the was a Lys-C digestion of were analyzed the mass to both and has a mass the the with the mass to the of and digested by Lys-C of the Lys-C digested coat protein by a was the mass of the the of or of and the the and the with was and by the and of the of the to of the with the to with the the of or the with the to a of the acid to or were the of the of and the of the has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid has been to the by the acid a overall mass of of by and a the of to a mass of the mass of mass of was by a of the the the to mass by with a mass has a mass of of the the has been the both or were a of to the and the mass were to or of the mass with the the the to the mass of a of the coat of the with mass mass of the by the overall of the and of of the the with mass a with mass a of the of the of the and to and the of the with the the the mass and with a has to a of to the acid of of the the with mass a of to a of of of a virus by a a to by a the coat protein a of the the been the virus was the mass was the the was was virus distinct the of to the the a mass a of to or was by mass and by the mass of by a were were carried out the mass mass the to the the coat protein of was the was to to to sequences to and a by mass spectrometry a of to by a acid to protein of mass was the of the the was by the the mass was the of the the was by the the a the was to the were was a of the After was and the virus the protein of a and a of the was to of was was protein were the of to the mass was a mass to of of the analyzed isolates. of a Lys-C was to with the to of the of and was by a of to a to the to was was to with to the to the mass the the the overall and to After the of by the of were the was to to and the a virus the mass and the amino acid of the coat been by a the and of proteins mass by amino acid sequences the been acid sequences of to by the to a complete of the coat protein the of electrospray ionization and matrix-assisted laser desorption/ionization of mass mass spectrometry a and virus coat protein sequences of the and the of of the and and of the and and of the and and of the and of the mass of the coat protein carried out with of a a of to coat proteins of or of the virus of the and by the protein with and the digestion products by the of the protein sequences with acid and the the amino acid mass spectrometry a by mass and to with the of a by a mass the of and the of amino acid sequences protein applied to a number of of the of has and and to the by and coat protein amino acid the with and to and of to and of to has the of and to the of of brome mosaic virus (BMV) of the and the of the coat protein of virus by mass spectrometry (TOFMS) of the and and the complete amino acid sequences of of and of the and and of the and and of the and to isolates. of to the by or of applied to virus a of of brome mosaic virus virus the of the and of and the and was of the to the of and has been and and and to of of the and of the protein the coat proteins of the the of the coat protein of the the acid of the and the of a number of the coat proteins carried out a of and digestion of the to the the the protein was to of and the to of the the of of amino of the and were the and and the the of the Lys-C were carried and the of the were by mass and the with the to the of the and and of the and and of and the were a the and mass the mass of the with the a was by the of a complete of with a complete of of the the the the of with of the coat protein of the the isolates. to the to determine the complete coat protein sequences of the of a Lys-C of the coat were by the mass and the by the of of the of a Lys-C of the coat was carried out a mass with with was the of a Lys-C of the coat protein by and of the of the a mass of the by the of the by the acid of the of the the of coat protein mass and the of with the with the of the of both and and and the with the mass of and the to with the overall mass a of and by the of and with a of the acid to with the the mass and and the to and of the and and of the and a with of the has the of the the the the was a Lys-C digestion of were analyzed the mass to both and has a mass the the with the mass to the of and digested by Lys-C of the Lys-C digested coat protein by a was the mass of the the of or of and the the and the with was and by the and of the of the to of the with the to with the the of or the with the to a of 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the overall and to After the of by the of were the was to to and of the and of the protein the coat proteins of the the of the coat protein of the the acid of the and the of a number of the coat proteins carried out a of and digestion of the to the the the protein was to of and the to of the the of of amino of the and were the and and the the of the Lys-C were carried and the of the were by mass and the with the to the of the and and of the and and of and the were a the and mass the mass of the with the a was by the of a complete of with a complete of of the the the the of with of the coat protein of the the isolates. to the to determine the complete coat protein sequences of the of a Lys-C of the coat was carried out a mass with with was the of a Lys-C of the coat protein by and of the of the a mass of the by the of the by the acid and of the protein the coat proteins of the the of the coat protein of the the acid of the and the of a number of the coat proteins carried out a of and digestion of the to the the isolates. the protein was to of and the to of the the of of amino of the and were the and and the the of the Lys-C were carried and the of the were by mass and the with the to the of the and and of the and and of and the were a the and mass the mass of the with the a was by the of a complete of with a complete of of the the the the of with of the coat protein of the the isolates. to the to determine the complete coat protein sequences of the of the of the the of coat protein mass and the of with the with the of the of both and and and the with the mass of and the to with the overall mass a of and by the of and with a of the acid to with the the mass and and the to and of the and and of the and a with of the has the of the the the the was a Lys-C digestion of were analyzed the mass to both and has a mass the the with the mass to of the Lys-C digested coat protein by a was the mass of the the of or of and the the and the with was and by the and of the of the 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was to with the to of the of and was by a of to a to the to was was to with to the to the mass the the the overall and to After the of by the of were the was to to and and the and and the and of virus

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