Effects of Estrogens on the Ultrastructural Characteristics of Female Rat Prolactin Cells as Evaluated by in situ Hybridization in Combination with Immunogold Staining Technique
Bibliographic record
Abstract
We have recently reported that, in the rat pituitary, prolactin (PRL) is synthesized and stored in two different cell types: the typical PRL cell characterized by the presence of large irregular secretory granules and another cell type containing small round secretory granules. Since the secretion of PRL is stimulated by estrogens, we decided to investigate the effect of gonadectomy and estradiol (E2) replacement therapy in female rats on both cell types using a combination of in situ hybridization and immunostaining. Gonadectomy induced a marked decrease in hybridization signal and an almost complete disappearance of the classical PRL cells. The most abundant PRL cells were the small-granule-containing cells and other PRL cells characterized by the presence of both small and large granules. These changes in the PRL cell population were completely prevented by E2 administration to castrated animals. These studies indicate that E2 cannot only regulate PRL mRNA but also induce important morphological changes in PRL cell types.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".