Comparison of Control Region Sequencing and Fragment RFLP Analysis for Resolving Mitochondrial DNA Variation and Phylogenetic Relationships among Great Lakes Walleyes
Bibliographic record
Abstract
Direct sequencing of 513 base pairs from the control region and restriction fragment length polymorphisms (RFLP) in two fragments totaling 7.6 kilobases (fragment RFLP) that were amplified by polymerase chain reaction were used to assess mitochondrial DNA (mtDNA) variation in Great Lakes walleye Stizostedion vitreum. Our objective was to determine the effectiveness of these mtDNA markers in detecting genetic variation and resolving phylogenetic relationships among haplotypes previously identified by RFLP analysis of the entire molecule. The fragment RFLP analysis surveyed 554 base pairs and detected almost twice as many haplotypes as did the sequencing analysis. However, both approaches resulted in similar tree topologies and resolved the three major phylogenetic assemblages published in entire-molecule RFLP studies.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".