Radical Formation at Tyr39 and Tyr153 Following Reaction of Yeast Cytochrome <i>c</i> Peroxidase with Hydrogen Peroxide
Bibliographic record
Abstract
The formation of yeast cytochrome c peroxidase (CcP) compound I has been recognized for many years to be associated with formation of two protein-centered radicals. One of these radical sites is located at Trp191 and is directly involved in catalytic oxidation of ferrocytochrome c (Sivaraja, M., Goodin, D. B., Smith, M., Hoffman, B. M. (1989) Science 245, 738-740). The second radical has been proposed to arise from one or more tyrosyl residues of CcP. However, the tyrosyl residue (or residues) capable of forming this radical has not been identified, and the functional role of this radical remains poorly understood. In the present work, this issue has been addressed through the combined use of the spin-trapping reagent 2-methyl-2-nitrosopropane and peptide mapping by electrospray mass spectrometry to identify Tyr39 and Tyr153 as two tyrosyl residues that are capable of forming radical centers upon reaction of CcP with hydrogen peroxide. The implications of this observation to the catalytic mechanism of CcP are addressed with reference to the three-dimensional structure of CcP.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".