Evaluation of deletion of nuclease genes cluster in L. major
Bibliographic record
Abstract
The nuclease is a surface enzyme unique to trypanosomatid parasites. These organisms lack the pathway for de novo purine biosynthesis and thus are entirely dependent upon their hosts to supply this nutrient for their survival, growth, and multiplication. There is a cluster on chromosome 30 which carries 2 copy of nuclease genes and 5 identical nuclease like proteins in L. major which are in cis form with 700-800 bp intergenic regions which have more than 80% homology. These data shows that this enzyme might play an important role in facilitating the survival, growth, and development of this important human pathogen. In previous studies, have been shown that L. major 3′NT/NU which is expressed specifically in promastigotes is not the key molecules involved in host purine salvaging pathway and thus in better understanding parasite strategies adopted to survive in sandflies. Therefore, only deletion of nuclease genes followed by sandfly infections experiments will allow determining its precise role in purine salvaging and sandfly infection and specificity. We have developed nuclease cluster heterozygote and homozygote knockout mutants, with homologous recombination technique, to evaluate deletion effects on survival of parasite and infection.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".