33 PRODUCTION OF CLONED BOER GOATS AND DORPER SHEEP IN ARGENTINA
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Somatic cell nuclear transfer (SCNT) has been proposed as an outstanding tool for expanding the dissemination capacity of animals of extreme genetic value, as well as for the genetic resurrection of elite animals affected by incurable disease or that died suddenly. Numbers of outstanding males of meat-specialised breeds of goats (Boer) and sheep (Dorper) recently imported into Argentina were expanded using SCNT technology. Oocytes were collected by laparoscopic ovum pickup (LOPU) from 40 Raza Criolla goats and 38 crossbreed sheep that were hormonally stimulated, as described previously (Baldassarre et al. 2002 Theriogenology 57, 275). Oocyte maturation, cell transfer, fusion and activation, culture, and transfer to recipients were conducted following procedures previously described (Baldassarre et al. 2003 Cloning Stem Cells 5, 279). Briefly, oocytes were matured in vitro for 24 h in TCM 199 supplemented with hormones and 10% serum, at 38.5°C and 5% CO2. 2 caprine fetal fibroblast cell lines (FF1 and FF2) were established from purebred Boer fetuses generated by selective breeding of elite animals, while a fibroblast ovine line was established from a skin biopsy from an elite Dorper ram. Cells were transferred into previously enucleated oocytes, followed by electric fusion using a single DC pulse of 1.6 kV cm–1 for 70 µs. Finally, the reconstructed embryos were activated using ionomycin (5 µM/5 min) followed by cycloheximide (10 µg mL–1) and cytochalasin B (7.5 µg mL–1) for 4 to 5 h, followed by in vitro culture in mSOF media before transfer into the oviducts of synchronized recipients within 24 h after fusion. An average of 10.4 and 12.8 reconstructed embryos were transferred to each of 21 and 12 recipient goats and sheep, respectively. Pregnancy was detected and monitored for the first 3 months by transrectal ultrasound scanning. Initial pregnancy (4 recipients, 33%) was maintained from gestation Day 30 to term in sheep, while goats exhibited a dramatic drop from 9 recipients pregnant (41%) on Day 30 to only 2 (9%) giving birth. Deliveries were by elective C-section. The number of normal offspring with good postpartum survival was 2/2 in goats (100%) and 3/5 (60%) in sheep. Substantial differences were observed between the 2 cell lines used in goats, where pregnancy was 4/11 (36%) for FF1 and 5/10 (50%) for FF2 at Day 30; however, only 2 goats carrying FF2 pregnancies carried. These results are in agreement with previous reports suggesting that cell line may be the largest source of result variation in SCNT. At the time of writing this abstract these clones are ~4 months of age, healthy and growing normally (>40 kg weight). To the best of our knowledge, these are the first cloned goats produced by SCNT technology in Latin America, and the second group to produce cloned sheep in the region.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it