Expression of a cDNA encoding palmitoyl‐acyl carrier protein desaturase from cat's claw (<i>Doxantha unguis‐cati</i> L.) in <i>Arabidopsis thaliana</i> and <i>Brassica napus</i> leads to accumulation of unusual unsaturated fatty acids and increased stearic acid content in the seed oil
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Bibliographic record
Abstract
Abstract A cDNA encoding palmitoyl‐acyl carrier protein (ACP)‐desaturase from cat's claw ( Doxantha unguis‐cati L.) was expressed in Arabidopsis thaliana and Brassica napus L. with the goal of decreasing the saturated fatty acid (FA) content of the seed oil. In general, transformation of Arabidopsis resulted in a greater change in the FA composition of the seed oil than for B. napus . An increase in palmitoleic acid (16:1 cis Δ 9 ) was obtained in transgenic lines, suggesting that the 16:0‐ACP‐desaturase cDNA was expressed in the manner originally intended. Other effects on lipid metabolism, however, were observed in the seed of transgenic plants. In Arabidopsis, there was a large increase in the proportions of cis ‐vaccenic acid (18:1 cis Δ 11 ) and cis ‐13‐eicosenoic acid (20:1 cis Δ 13 ), possibly generated through elongation of 16:1 cis Δ 9 . Elongation of 18:1 cis Δ 11 to 20:1 cis Δ 13 , however, was not observed in B. napus indicating that certain aspects of lipid metabolism in the model plant, Arabidopsis, may not apply to B. napus . As well, the appearance of 18:1 cis Δ 11 was accompanied by a decrease in the proportion of oleic acid (18:1 cis Δ 9 ). Although the introduced ACP‐desaturase resulted in synthesis of some unsaturated FAs, the overall saturated FA content was maintained at similar levels to the control or was enhanced. Increased levels of saturation were mainly associated with an increase in stearic acid, which unlike 16:0, is considered non‐atherogenic. The results suggest that a mechanism exists further downstream in oil biosynthesis to counteract the decrease in saturation brought about by the 16:0‐ACP‐desaturase action.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it