Regulation of the Activity of N-1-Naphthylphthalamic Acid Binding Protein by ATP and Phosphatase
Why this work is in the frame
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Bibliographic record
Abstract
N-1-naphthylphthalamic acid (NPA), an auxin transport inhibitor, was found to bind specifically to a crude membrane preparation from sugar beet seedling leaf cell suspension cultures. The dissociation constant (Kd) and binding protein concentration were found to be 1.71 μmol dm-3 and 220 pmol g-1(membrane protein), respectively. The amount of specific 3H-NPA binding was significantly increased by adding Mg2+ATP to the binding assay solution. Treatment of membrane preparations with acid phosphatase, prior to the NPA binding assay, resulted in lower specific binding. ATP activation and phosphatase inactivation were culture stage dependent. Although a considerable effect could be detected when using cells from day 8 (representing the linear phase), the same treatment did not alter the binding if cells from day 1 (representing lag phase) or day 14 (representing the stationary phase) were used. These observations have strongly highlighted the possible involvement of a phosphorylation and dephosphorylation mechanism in vivo in the regulation of the activity of the NPA binding protein. High phosphatase activity was found in the supernatant, but not in the membrane pellet) after 50 000 g centrifugation. Our present study has indicated that receptor activity could be regulated by a phosphorylation and dephosphorylation mechanism in plants.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it