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Record W2094143038 · doi:10.2135/cropsci2011.07.0363

Consensus Mapping and Identification of Markers for Marker‐Assisted Selection of <i>Wsm2</i> in Wheat

2012· article· en· W2094143038 on OpenAlexfundno aff
Huangjun Lu, Rebecca A. Kottke, Ravindra N. Devkota, Paul St. Amand, Amy Bernardo, Guihua Bai, Patrick F. Byrne, Terry J. Martin, Scott D. Haley, Jackie C. Rudd

Bibliographic record

VenueCrop Science · 2012
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicPlant Virus Research Studies
Canadian institutionsnot available
FundersCanadian Association of Palynologists
KeywordsBiologyMarker-assisted selectionGenetic markerGeneticsGenotypePopulationPolymerase chain reactionAlleleAncestry-informative markerMolecular markerAllele frequencyGene

Abstract

fetched live from OpenAlex

ABSTRACT A recently identified Wheat streak mosaic virus (WSMV) resistance gene Wsm2 confers a high level of resistance. Objective of this study was to identify closely linked DNA markers for Wsm2 for use in marker‐assisted selection (MAS) in wheat ( Triticum aestivum L.). Two segregating populations (CO960293‐2 × ‘TAM 111’ and CO960293‐2 × ‘Yuma’) of F 2:3 families were evaluated for response to WSMV infection in growth chamber experiments. Forty‐eight simple sequence repeat (SSR) or sequence‐tagged site (STS) markers were screened for polymorphism between the parents of both populations. In the CO960293‐2 × TAM 111 population, five markers were mapped to the region of Wsm2 with XSTS3B‐55 being the closest marker (5.2 cM distal to Wsm2 ). In the CO960293‐2 × Yuma population, eight markers were linked to Wsm2 with the closest marker Xbarc102 linked at 3.9 cM proximal to Wsm2 . Results from consensus mapping of the two populations suggested that Xbarc102 was distal to Wsm2 . The marker Xbarc102 was associated with Wsm2 in all 22 wheat lines derived from crosses between susceptible parents and either CO960293‐2 or ‘RonL’ (also carrying Wsm2 ). The marker allele Xbarc102‐219‐bp present in CO960293‐2 was amplified in polymerase chain reaction (PCR) from Wsm2 ‐carrying genotypes CO960293‐w133, RonL, and ‘Snowmass’ but not from the resistant line KS96HW10‐3 (carrying Wsm1 ) or the susceptible genotypes ‘Karl 92’, ‘TAM 107’, and ‘N96L9970’. Therefore, this marker should be useful for MAS of Wsm2 in breeding programs.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.788
Threshold uncertainty score0.179

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.057
GPT teacher head0.291
Teacher spread0.235 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations27
Published2012
Admission routes1
Has abstractyes

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