Retracted: Isocratic Method for Affinity Enrichment of Covalently‐linked Peptides in Cyanogen Bromide Cleavage of Proteins
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Post-publication record
- Nature
- Retraction
- Reason
- Duplication of/in Article;False/Forged Affiliation;
- Date
- 9/20/2011 0:00
- Flagged by OpenAlex?
- Yes
Source: Retraction Watch, joined by DOI. OpenAlex records retraction as is_retracted, a boolean over a state space with at least four values, so it cannot express an expression of concern, a correction or a reinstatement — it reports them as false, which reads as “fine”.
Abstract
The low resolution three-dimensional structure of a protein can be inferred from existing disulfide bridges or experimentally introduced chemical crosslinks. The general procedure involves enzymatic digestion of a protein followed by mass spectrometry-based identification of covalently-linked peptides, native disulfide-linked peptides and chemically cross-linked peptides. To facilitate unambiguous identification of these peptides, an isocratic purification method was developed for selective enrichment of covalently-linked cyanogen bromide (CNBr) fragments. This method capitalizes on the ability of homoserine lactone moieties at the C-termini of CNBr cleavage products for selective conjugation of primary-amine containing affinity tag. The availability of two C-termini within covalently-linked peptides allows for the conjugation of two affinity tags, whereas the other peptides have only one affinity tag at the C-terminus, which enables selective enrichment of covalently-linked peptides by utilization of affinity tag with moderate dissociation constant. Here we demonstrate successful implementation of this method with tetrahistidine as the affinity tag for enrichment of covalently-linked CNBr fragments of test peptides and proteins.
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The record
- Venue
- PROTEOMICS
- Topic
- Advanced Proteomics Techniques and Applications
- Field
- Chemistry
- Canadian institutions
- Occupational Cancer Research CentreUniversity of Toronto
- Funders
- —
- Keywords
- Cyanogen bromideCovalent bondCleavage (geology)BromideChemistryProteomicsPosttranslational modificationAffinity chromatographyCombinatorial chemistryPeptideStereochemistryBiochemistryPeptide sequenceOrganic chemistryBiologyEnzyme
- Has abstract in OpenAlex
- yes