Temperature-Dependence of L-Type Ca2+ Channel Current in Atrial Myocytes From Rainbow Trout
Bibliographic record
Abstract
Rainbow trout, Oncorhynchus mykiss, inhabit eurythermal environments and must therefore be able to cope with changes in environmental temperature. As ectotherms, their heart is required to maintain cardiac function over a range of ambient water temperatures. This raises important questions concerning the temperature-dependence of cardiac ion channel function in fish hearts, in particular, the channels involved in Ca(2+) transport. Thus, we studied the effects of acute, physiologically relevant temperature changes on the density and kinetics of the L-type Ca(2+) channel current (I(Ca)) in rainbow trout atrial myocytes using the whole-cell patch-clamp technique. Myocytes from fish acclimated to 14 degrees C were first tested at 14 degrees C, then at 21 degrees C and finally at 7 degrees C. Using a square-pulse voltage-clamp in the first series of experiments, the peak density of I(Ca) increased (Q(10)=1.9) as temperature was increased from 14 to 21 degrees C and decreased (Q(10)=2.1) as temperature was decreased from 14 to 7 degrees C. In contrast to current density, the charge carried by I(Ca) was inversely related to temperature as a result of changes in the kinetic properties of the channel; both the fast (tau(f)) and slow (tau(s)) components of inactivation were slower at 7 degrees C than at 14 and 21 degrees C. Action potentials were recorded at the three test temperatures and then used as voltage-clamp stimulus waveforms to reassess I(Ca) in a second series of experiments. While the temperature-dependency of I(Ca) was similar to that found with the square-pulse voltage-clamp, the charge carried by I(Ca) was temperature-independent. These results show that the temperature-dependency of I(Ca) in rainbow trout is in the lower range of that reported in mammals and, although this could have profound effects on Ca(2+) delivery to the myofilaments, the temperature-induced modifications in the action potential may help to maintain a fairly constant Ca(2+) delivery during an acute temperature change in rainbow trout.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".