DOMOIC ACID PRODUCTION By <i>PSEUDO‐NITZSCHIA SERIATA</i> (BACILLARIOPHYCEAE) IN SCOTTISH WATERS<sup>1</sup>
Why this work is in the frame
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Bibliographic record
Abstract
In 1999, a 49,000 km 2 area in western Scottish waters was closed to shellfish harvesting due to the amnesic shellfish poisoning (ASP) toxin domoic acid (DA). The only previously confirmed DA producer identified had been Pseudo‐nitzschia australis Frenguelli. The toxin has appeared every year since and has led to more harvesting closures. We isolated and cultured two strains of Pseudo‐nitzschia seriata f. seriata (P. T. Cleve) H. Peragallo from western Scottish waters in 2001 and 2002. They were identified using TEM analysis of their morphological fine structure and sequencing of the internal transcribed spacer (ITS)1, 5.8S, ITS2, and partial large subunit (LSU) rDNA. The morphology of the Scottish P. seriata f. seriata strains differed slightly, for example, in the number of poroid rows, from descriptions in identification keys. Comparison of P. seriata sequences with those of two co‐occurring Pseudo‐nitzschia australis isolates showed an overall divergence of only 0.012. Sequence divergence between both species was highest in the ITS1 region (0.036). Combined morphological and genetic approaches are needed to identify closely related Pseudo‐nitzschia species. The P. seriata strains grew successfully at 15°C, suggesting that although seen as a psychrophilic species, it may also occur at higher water temperatures. All isolates produced DA in stationary phase (measured on day 25): 0.16–0.23 pg DA·cell −1 in P. seriata and 0.15–1.68 pg DA·cell −1 in P. australis . Our study is the first to identify P. seriata f. seriata as a DA producer in Scottish waters and indicates that at least it and P. australis can be responsible for ASP toxicity in that region.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it