Cloning and homology modeling of a serine protease gene (PrC) from the nematophagous fungus Clonostachys rosea
Bibliographic record
Abstract
The nematophagous fungus Clonostachys rosea can parasitize nematodes as well as insects and several fungi. Proteases play critical roles during infection and are considered important virulence factors in these fungi. In this study, a cuticle-degrading serine protease gene ( PrC ) was cloned for the first time from C. rosea . The gene contained three introns and four exons and encodes a polypeptide of 386 amino acid residues. The mature protein is 277 amino acid residues long and contains a conserved motif shared by peptidase S8 family members. Its N-terminal amino acid residues showed a high degree of sequence similarity with serine proteases from nematophagous and entomopathogenic fungi. Based on the PrC amino acid sequence, the three-dimensional structure has been predicted and compared with that of protease K. Our results provide a basis for further understanding the molecular mechanism of C. rosea infection of nematodes. Such knowledge could be explored for improving by genetic engineering the effectiveness of the use of fungal infections to control parasitic nematodes.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".