Enriched stable isotope marking of juvenile golden perch (<i>Macquaria ambigua</i>) otoliths
Bibliographic record
Abstract
Stocking of native fish is a standard practice to aid in the recovery and enhancement of depleted populations. However, evaluating the effectiveness of these stocking programs has been hindered because of difficulty in distinguishing hatchery from wild fish, especially for species that are stocked as small, fragile fingerlings. Stable isotopes offer the potential to chemically mark fish with multiple marks that are distinct from each other and from natural signatures. Golden perch (Macquaria ambigua) fingerlings were reared in water enriched in 137 Ba and combinations of 137 Ba and 86 Sr for various lengths of time. Ba and Sr isotopic ratios in the otoliths were determined using laser ablation inductively coupled plasma-mass spectrometry. Fish exposed to increased levels of 137 Ba had lower 138 Ba/ 137 Ba ratios in their otoliths relative to the natural ratio of control fish and were significantly different in fish exposed to at least 5 µg·L -1 for 8 days or to 15 µg·L -1 for 4 days. Furthermore, eight unique signatures were produced in fish reared in combinations of 137 Ba (0–5 µg·L -1 ) and 86 Sr (0–100 µg·L -1 ) for 24 days. Our results suggest that immersion in water enriched in specific stable isotopes could be an effective means of marking hatchery-reared fish for stock enhancement.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.002 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".