Direct Recording of Renal Sympathetic Nerve Activity in Unrestrained, Conscious Mice
Bibliographic record
Abstract
Renal sympathetic nerve activity (RSNA) has been measured in anesthetized mice. However, anesthesia and acute surgical preparation cause poor cardiovascular stability and unphysiological blood pressures. This compromised physiological state confounds proper interpretation of experimental results considering the inseparable link between cardiovascular status and autonomic nervous tone. We, therefore, developed a surgical and experimental protocol for measuring RSNA in conscious, unrestrained mice. Male C57Bl/6J mice were chronically instrumented with blood pressure radiotelemeters, an indwelling jugular venous catheter and a bipolar electrode for recording RSNA. Mice were placed in a home cage and left to recover for 48 to 72 hours. Survival rate was 100%; all of the mice exhibited normal behavior with no sign of distress 24 hours after surgery. RSNA was successfully recorded in 80% of the mice at 48 and 72 hours postsurgery; viable RSNA was reduced to 70% and 50% at 4 and 5 days postsurgery, respectively. Mean arterial pressure (116±2 mm Hg; n=10) was consistent with values reported previously for conscious mice. RSNA increased with the normal physical activities of eating and grooming and was validated by ganglionic blockade and pharmacological manipulation of blood pressure; reduction in blood pressure to 62±3 mm Hg with nitroprusside increased RSNA by 77±9% above baseline (n=5; P<0.05), whereas an increase in blood pressure to 137±6 mm Hg with phenylephrine reduced RSNA by 79±2% compared with baseline (n=5; P<0.05). Thus, we demonstrate an accessible and effective method for direct assessment of RSNA in conscious, unrestrained mice.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".