Localization of Guanylate Cyclase Receptor, Inositol Trisphosphate Receptor, and Calmodulin in Boar Spermatozoa
Bibliographic record
Abstract
Several proteins present on the sperm membranes serve as signal transducers facilitating sperm maturation, capacitation,acrosome reaction and fertilization. Among them, guanylate cyclase (GC), the inositol trisphosphate receptor (IP3R) andcalmodulin have been reported rather extensively. Although biochemical data clearly suggest their roles in thefertilization process, the precise location of these proteins in the sperm membranes have not been adequately investigatedand need clarification. Using immunolocalization procedures we report that guanylate cyclase receptors (GCR) arelocalized on the apical region of the acrosome and on the postacrosomal plasmalemma, while IP3Rs are present in theneck region of the sperm and at lower density along the axonemal membrane. In contrast, calmodulin is restricted to thepostacrosomal, basal nuclear membrane and the neck region that corresponds to the redundant nuclear envelope and ERvesicles, with some reaction along the middle piece and tail membranes. Colocalization of these proteins on the spermmembrane together with biochemical data as reported previously provide further insight into their role in thespermatozoan motility and possibly the fertilization process.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".