Universal primer cocktails for fish DNA barcoding
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Machine scores (provisional)
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
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- Teacher spread
- 0.263 · how far apart the two teachers sit on this one work
- Validation status
score_only:v0-immature-baseline· verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it
Abstract
Abstract Reliable recovery of the 5′ region of the cytochrome c oxidase 1 (COI) gene is critical for the ongoing effort to gather DNA barcodes for all fish species. In this study, we develop and test primer cocktails with a view towards increasing the efficiency of barcode recovery. Specifically, we evaluate the success of polymerase chain reaction amplification and the quality of resultant sequences using three primer cocktails on DNA extracts from representatives of 94 fish families. Our results show that M13‐tailed primer cocktails are more effective than conventional degenerate primers, allowing barcode work on taxonomically diverse samples to be carried out in a high‐throughput fashion.
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The record
- Venue
- Molecular Ecology Notes
- Topic
- Identification and Quantification in Food
- Field
- Biochemistry, Genetics and Molecular Biology
- Canadian institutions
- University of Guelph
- Funders
- Natural Sciences and Engineering Research Council of CanadaCanada Research ChairsOntario Innovation TrustOntario Genomics InstituteGenome CanadaGordon and Betty Moore Foundation
- Keywords
- Primer (cosmetics)BiologyDNA barcodingBarcodePolymerase chain reactionComputational biologyGeneticsFisheryGeneEvolutionary biologyComputer scienceChemistry
- Has abstract in OpenAlex
- yes