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Record W2104294761 · doi:10.1101/cshperspect.a002204

Closing the Circle: Replicating RNA with RNA

2010· review· en· W2104294761 on OpenAlexaff
Lü Cheng, Peter J. Unrau

Bibliographic record

VenueCold Spring Harbor Perspectives in Biology · 2010
Typereview
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicRNA and protein synthesis mechanisms
Canadian institutionsSimon Fraser University
Fundersnot available
KeywordsBiologyRibozymeRNA world hypothesisRNAComputational biologyGeneticsLigase ribozymeAbiogenesisEvolutionary biologyNon-coding RNAGene

Abstract

fetched live from OpenAlex

How life emerged on this planet is one of the most important and fundamental questions of science.Although nearly all details concerning our origins have been lost in the depths of time, there is compelling evidence to suggest that the earliest life might have exploited the catalytic and self-recognition properties of RNA to survive.If an RNA based replicating system could be constructed in the laboratory, it would be much easier to understand the challenges associated with the very earliest steps in evolution and provide important insight into the establishment of the complex metabolic systems that now dominate this planet.Recent progress into the selection and characterization of ribozymes that promote nucleotide synthesis and RNA polymerization are discussed and outstanding problems in the field of RNA-mediated RNA replication are summarized.C ell division is a fundamental biological process in which genetic information is duplicated and shared between daughter cells.In extant cellular life, DNA serves as the repository of genetic information, but its replication is complicated by the daunting size and complex structural organization of modern genomes.For this reason, multiple enzymes are required to ensure faithful genomic replication in all higher life forms.Notably, simpler replicating systems such as viruses, have smaller genomes and tend to use correspondingly more errorprone replicative machinery (Kunkel and Bebenek 2000;Gago, Elena et al. 2009).Presumably, if the initial organisms on this planet also had small genomes, then the earliest genomic replication could have been a relatively simple and error-prone process compared with the complex replicative strategies of modern life.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Review · Consensus signal: Review
Teacher disagreement score0.004
Threshold uncertainty score0.014

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0020.002
Science and technology studies0.0010.002
Scholarly communication0.0020.004
Open science0.0010.001
Research integrity0.0020.004
Insufficient payload (model declined to judge)0.0040.009

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.023
GPT teacher head0.310
Teacher spread0.287 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreReview

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations57
Published2010
Admission routes1
Has abstractyes

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