Recovery of Astaxanthin from<i>Paracoccus</i>NBRC 101723 using Ultrasound-Assisted Three Phase Partitioning (UA-TPP)
Why this work is in the frame
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Bibliographic record
Abstract
Astaxanthin (AX) is a high valued ketocarotenoid having numerous applications in the food and pharmaceutical industry. The present study is a combined approach of using ultrasound extraction with three phase partitioning for an efficient recovery of Astaxanthin from Paracoccus NBRC 101723. The optimum conditions for the ultrasonic extraction of AX were: 100% amplitude for 20 s (wet biomass) and 60 s (dried biomass) using solid to solvent ratio (1:2) at a distance of 15 mm between the base of the extraction vessel and the tip of the probe. Pretreatment of the dried biomass (particle size 0.8 μm) with 70% acetone (70°C, 25 min) resulted in maximum ultrasonic extraction of 1035 μg/g of dried biomass. The astaxanthin content (μg/g) extracted using dried biomass, dried using different drying methods showed hot air oven drying at 35°C (946 ± 23) to be comparable to freeze drying (950 ± 19) and vacuum oven drying (945 ± 22). Astaxanthin from the disrupted cells recovered by three phase partitioning using (NH4)2SO4 and t-butanol (40°C, 30 min) resulted in 37% more recovery than conventional method.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it