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Record W2105021824 · doi:10.3920/wmj2010.1224

Comparison of monoclonal antibody performance characteristics for the detection of two representatives of A- and B-trichothecenes: T-2 toxin and deoxynivalenol

2010· article· en· W2105021824 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

fundA Canadian funder is recorded on the work.
no affNo Canadian affiliation: this work is invisible to an affiliation-only frame.
No Canadian affiliation. An affiliation-only frame, the usual design, would never have seen this work. It is one of the works that make the case for inverting the frame.

Bibliographic record

VenueWorld Mycotoxin Journal · 2010
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicMycotoxins in Agriculture and Food
Canadian institutionsnot available
FundersQueen's UniversityQueen's University BelfastEuropean Commission
KeywordsTrichotheceneToxinMycotoxinDiacetoxyscirpenolMonoclonal antibodyFusariumBiologyVomitoxinZearalenoneMicrobiologyAspergillusAntibodyFood scienceImmunologyBotany

Abstract

fetched live from OpenAlex

Mycotoxins are secondary metabolites produced by fungi belonging mainly to the Aspergillus, Penicillium and Fusarium genera. They represent a relevant source of danger to human and animal health causing food- and feedborne intoxication. One group, produced by Fusarium spp., are the trichothecenes, of which T-2 toxin belongs to the type-A trichothecenes and deoxynivalenol to the type-B trichothecenes. As these mycotoxins are ubiquitous, the testing of products is required to keep our food and feed safe. For this purpose, sensitive and reliable tests are needed to detect contaminations. One detection possibility is an immunoanalytical based test which needs antibodies as reagents. Cell culture facilities allow cell line selection and production of monoclonal antibodies for further immunological test development. Especially for mycotoxins antibody development for further use in immunoassays is a crucial task. T-2 toxin and deoxynivalenol specific monoclonal antibodies were developed and further characterised to test stability and solvent resistance properties. Especially cross-reactivities were determined to related mycotoxins also belonging to the trichothecene family, e.g. HT-2 toxin or 3-acetyldeoxynivalenol.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.430
Threshold uncertainty score0.162

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.026
GPT teacher head0.314
Teacher spread0.288 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it