Analysis of Na+, Cl-, K+, H+and NH4+ concentration gradients adjacent to the surface of anal papillae of the mosquito<i>Aedes aegypti</i>: application of self-referencing ion-selective microelectrodes
Bibliographic record
Abstract
Ion concentration gradients adjacent to the surface of the anal papillae of larvae of the mosquito Aedes aegypti were measured using self-referencing ion-selective microelectrodes. The gradients were used to calculate estimates of ion fluxes into and out of the papillae. There was a net influx of Na+, Cl- and K+ from the bathing medium and a net efflux of acid and NH4+. No Ca2+ gradients were detectable. Na+ and Cl- influx occurred against a concentration gradient suggesting active transport. Although Na+, Cl- and NH4+ gradients were uniform along the length of the papillae, the proximal regions of the papillae in vivo revealed significantly higher H+ and K+ gradients compared with distal regions. The calculated ion fluxes at the papillae are sufficient for complete Na+, K+ and Cl- haemolymph replacement in approximately 4 h with external ion concentrations of 5 mmol l(-1). Ion gradients were also detected adjacent to the surface of isolated papillae; however, Na+ and H+ gradients were higher, and Cl- gradients were lower relative to papillae in vivo. The results support previous findings that the anal papillae of mosquito larvae are important structures for ion regulation, and suggest that these structures may be used for the excretion of nitrogenous waste.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".