Cloning, expression and localization of a trypsin‐like serine protease in the spruce budworm, <i>Choristoneura fumiferana</i>
Why this work is in the frame
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Bibliographic record
Abstract
Abstract A trypsin‐like molting‐related serine protease cDNA ( Cf MRSP) was cloned from the spruce budworm, Choristoneura fumiferana . The full‐length Cf MRSP complementary DNA (cDNA) encoded a 43 kDa protein that contained a trypsin‐like serine protease catalytic domain, but no clip domain. The C‐terminal extension contained five cystein residues, which may allow the protein to form a homodimer through interchain disulfide bonds and regulate the activity of Cf MRSP. Phylogenetic tree analysis showed that Cf MRSP clusters with lepidopteran homologues such as serine protease 1 of Lonomia obliqua , hemolymph proteinase 20 (HP20), pattern recognition serine proteinase precursor (ProHP14) and a trypsin‐like protein of Manduca sexta . Northern blot analysis of developmental expression of Cf MRSP indicated that its transcripts were found primarily in the epidermis and were produced during all of the tested stadia, from 4th instar larvae to pupae, but increased levels of Cf MRSP transcripts were always found after each molt. A high level of the protein was found in the epidermis by immunohistochemistry analysis. Altogether these data suggest that Cf MRSP plays a role in the epidermis during molting and metamorphosis.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it