MétaCan
Menu
Back to cohort
Record W2123732614 · doi:10.1093/molehr/gam051

Region-specific gene expression profiling along the human epididymis

2007· article· en· W2123732614 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueMolecular Human Reproduction · 2007
Typearticle
Languageen
FieldMedicine
TopicSperm and Testicular Function
Canadian institutionsUniversité LavalCentre hospitalier de l'Université Laval
FundersCanadian Institutes of Health Research
KeywordsEpididymisBiologyGene expression profilingGeneGene expressionTranscriptomeSpermMicroarray analysis techniquesNorthern blotDNA microarrayGeneticsGene chip analysisMicroarrayComputational biologyCell biology

Abstract

fetched live from OpenAlex

During their transit through the epididymis, spermatozoa undergo many biochemical modifications necessary to acquire flagellar motility and fertilizing ability. These modifications, collectively called sperm maturation, are well orchestrated along the epididymis and depend on highly regionalized gene expression patterns. Based on clinical observations, the role of the epididymis in human sperm maturation has been questioned. To further understand the function of the excurrent duct in humans, we analysed gene expression of three donors on 'Affymetrix human GeneChip U133 plus 2' representing 47,000 transcriptional variants. More than 50% of transcripts were detected in each epididymal region. The analysis of hierarchical clustering performed from 2274 modulated qualifers between the three regions revealed that 1184, 713 and 269 were highly expressed in the caput, corpus and cauda region, respectively, in a very specific manner. The expressed qualifers were grouped according their similarity by Gene Ontology to give an overview of the functional features of the encoded proteins and to elucidate their potential roles in the epididymis. Northern blot analysis of eight gene transcripts predicted by microarray data to be highly expressed in the human epididymis was performed. All the transcript expression patterns confirmed the microarrays results. The data generated in this study demonstrate a region-specific gene expression pattern along the human epididymis that seems to coincide with the morphologically distinctive features of the excurrent duct.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.042
Threshold uncertainty score0.625

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0010.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.034
GPT teacher head0.284
Teacher spread0.249 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it