Effects of atrial natriuretic peptide on the extrasplenic microvasculature and lymphatics in the rat <i>in vivo</i>
Bibliographic record
Abstract
We developed a novel model using fluorescent intravital microscopy to study the effect of atrial natriuretic peptide (ANP) on the extrasplenic microcirculation. Continuous infusion of ANP into the splenic artery (10 ng min(-1) for 60 min) of male Long-Evans rats (220-250 g, n = 24) induced constriction of the splenic arterioles after 15 min (-7.2 +/- 6.6% from baseline diameter of 96 +/- 18.3 microm, mean +/- S.E.M.) and venules (-14.4 +/- 4.0% from 249 +/- 25.8 microm; P < 0.05). At the same time flow did not change in the arterioles (from 1.58 +/- 0.34 to 1.27 +/- 0.27 ml min(-1)), although it decreased in venules (from 1.67 +/- 0.23 to 1.15 +/- 0.20 ml min(-1)) and increased in the lymphatics (from 0.007 +/- 0.001 to 0.034 +/- 0.008 ml min(-1); P < 0.05). There was no significant change in mean arterial pressure (from 118 +/- 5 to 112 +/- 5 mmHg). After continuous ANP infusion for 60 min, the arterioles were dilated (108 +/- 16 microm, P < 0.05) but the venules remained constricted (223 +/- 24 microm). Blood flow decreased in both arterioles (0.76 +/- 0.12 ml min(-1)) and venules (1.03 +/- 0.18 ml min(-1); P < 0.05), but was now unchanged from baseline in the lymphatics (0.01 +/- 0.001 ml min(-1)). This was accompanied by a significant decrease in MAP (104 +/- 5 mmHg; P < 0.05). At 60 min, there was macromolecular leak from the lymphatics, as indicated by increased interstitial fluorescein isothiocyanate-bovine serum albumin fluorescence (grey level: 0 = black; 255 = white; from 55.8 +/- 7.6 to 71.8 +/- 5.9, P < 0.05). This study confirms our previous proposition that, in the extrasplenic microcirculation, ANP causes greater increases in post- than precapillary resistance, thus increasing intrasplenic capillary hydrostatic pressure (P(c)) and fluid efflux into the lymphatic system. Longer-term infusion of ANP also increases Pc, but this is accompanied by increased 'permeability' of the extrasplenic lymphatics, such that fluid is lost to perivascular third spaces.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".