HIGH‐PRESSURE DESTRUCTION KINETICS OF SPOILAGE AND PATHOGENIC BACTERIA IN RAW MILK CHEESE
Why this work is in the frame
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Bibliographic record
Abstract
ABSTRACT Raw milk cheese samples were individually inoculated with Escherichia coli K‐12, Escherichia coli O157:H7 and Listeria monocytogenes, and subjected to high‐pressure (HP) treatments (200–400 MPa) in both pressure hold and pressure pulse modes. Pressure inactivation of E. coli K‐12 at 350 MPa (5 min) at different temperatures demonstrated a strong temperature effect when temperature was 40C and above. Pressure destruction kinetics and pressure sensitivity were evaluated at 25C (where the temperature effect was minimal). HP treatment generally demonstrated a step‐change pressure pulse effect followed by a holding time destruction that was well described by a first‐order model ( R 2 > 0.90) with higher pressures resulting in a faster rate of microbial reduction (smaller D values). The associated D values at an intermediate pressure of 300 MPa were 4.4, 14.5 and 3.6 min for E. coli K‐12, E. coli O157:H7 and L. monocytogenes, respectively, with E. coli O157:H7 thus demonstrating a higher‐pressure resistance than the other two. The corresponding pressure z p values were 156, 128 and 82 MPa, and Δ V ≠ (Arrhenius volume change of activation) values were − 3.6, − 4.4 and − 7.0 ( × 10 −5 m 3 /mole), respectively. Pulse mode treatments showed similar pressure resistance trends to pressure hold mode, but were not effective for E. coli O157:H7.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it