Polymorphic microsatellite loci for<i>Littorina plena</i>show no population structure between the eastern and western coasts of Vancouver Island, Canada
Bibliographic record
Abstract
Marine snails of the genus Littorina are found on rocky intertidal shores throughout the north Atlantic and north Pacific oceans and comprise 19 species (Reid, 1996). This genus is commonly used for population genetic studies; Littorina species typically have separate sexes, moderately short generation times, low adult dispersal rates and large intertidal populations, making them convenient for comparative genetic studies (Berger, 1973; Kyle & Boulding, 2000; Lee & Boulding, 2009). Microsatellite markers have been developed for several Littorina species (Tie, Boulding & Naish, 2000; Sokolov et al., 2003; McInerney et al., 2009), but none have been developed for L. plena (Gould, 1849) or its sister species, L. scutulata (Gould, 1849). In this study, primers for microsatellite loci were initially developed from a genomic library for L. plena and were subsequently tested for cross-species amplification in six other Littorina species: L. scutulata, L. littorea (Linnaeus, 1758), L. sitkana Philippi, 1846, L. subrotundata (Carpenter, 1864), L. obtusata (Linnaeus, 1758) and L. saxatilis (Olivi, 1792). Amplification success in related species was expected to decrease with increasing evolutionary distance (Primmer et al., 2005; Panova et al., 2008). Therefore we expected that L. plena microsatellite primers would also amplify loci in its sister species L. scutulata (Reid, Rumbak & Thomas, 1996) and possibly in Littorina species closely related to that clade.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".