Ovarian Stanniocalcin in Trout Is Differentially Glycosylated and Preferentially Expressed in Early Stage Oocytes1
Bibliographic record
Abstract
The stanniocalcin (STC) gene was recently found to be widely expressed in fish. In this study, we have characterized ovarian STC in the rainbow trout (Oncorhynchus mykiss) and cloned the ovarian cDNA. The STC gene expression was highest in early stage oocytes and diminished progressively as oocytes developed. At the cellular level, ovarian STC gene expression was most abundant in the ooplasm of early stage oocytes, but it was also weakly evident in the theca layer, interstitial cells, and vitellogenic oocytes. The STC protein was distributed in a pattern similar to that of gene expression but was also apparent in glycoprotein vesicles, nuclei, multivesicular bodies, and follicles undergoing atresia. Cloned cDNAs obtained from the corpuscles of Stannius (CS) and ovarian transcripts were nearly identical. However, Western blotting of the partially purified proteins revealed that ovarian STC was larger than CS STC. Further analysis revealed that ovarian STC had a much larger N-linked carbohydrate moiety (approximately 12 kDa) compared to CS STC (approximately 7 kDa), indicating that the two hormones were differentially posttranslationally modified. To our knowledge, this is the first characterization of STC gene expression, cDNA, and protein distribution in the piscine ovary and the first evidence for any difference between alternative sources of the hormone in any species.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".