Crystal structure of methenyltetrahydrofolate synthetase from <i>Mycoplasma pneumoniae</i> (GI: 13508087) at 2.2 Å resolution
Bibliographic record
Abstract
Introduction. The enzyme 5,10-methenyltetrahydrofolate synthetase (MTHFS), also known as 5-formyltetrahydrofolate cyclo-ligase, is involved in folate metabolism. MTHFS catalyzes the obligatory initial metabolic step in the intracellular irreversible conversion of 5-formyltetrahydrofolate to other reduced folates, which requires ATP and magnesium. The product is then interconverted into other reduced folates involved in one-carbon metabolism. The reduced tetrahydrofolates (THFs) serve as cofactors that carry one-carbon moieties for the de novo synthesis of purines (supply the number 2 and number 8 carbons of the purine ring) and thymidylate (used for the methylation of dUMP to dTMP), and for the remethylation of homocysteine to methionine. 6] MTHFS from Mycoplasma pneumoniae shares significant sequence homology with human MTHFS. Although sequence information for MTHFS from various organisms is available, no structural information for this family of enzymes has been obtained. To provide a structural basis for understanding the function of the MTHFS protein family, we have determined the crystal structure of M. pneumoniae MTHFS at 2.2 resolution.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.002 | 0.001 |
| Open science | 0.002 | 0.000 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.009 | 0.003 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".