DNA fingerprinting of tabanids (Diptera: Tabanidae) and their respective egg masses using PCR – restriction fragment profiling
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Abstract Polymerase chain reaction and subsequent restriction fragment profiling analysis were used to associate collected tabanid egg masses with their respective species of adult horse flies and deer flies (Diptera: Tabanidae) in Manitoba, Canada. The ribosomal DNA (rDNA) intergenic spacer between the 28S and 18S ribosomal RNA genes was used successfully to differentiate 34 species of adult tabanids representing five genera: Atylotus (1 sp.), Chrysops (10 spp.), Haematopota (1 sp.), Hybomitra (17 spp.), and Tabanus (5 spp.). rDNA was a suitable molecular target for identifying tabanid species because of the high level of interspecific variation when comparing fragment profiles among different species, and the corresponding minimal intraspecific variation among individuals of the same species. Restriction fragment profiles from 56 field-collected tabanid egg masses were compared with those previously obtained from adults of known species. Egg masses of five species were identified: Hybomitra lasiophthalma (Macquart), Hybomitra nitidifrons nuda (McDunnough), Chrysops aestuans van der Wulp, Chrysops excitans Walker, and Chrysops mitis Osten Sacken. We also provide physical descriptions of these tabanid egg masses along with pictures.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it