Wiring efficiency of a metallizable DNA linker for site-addressable nanobioelectronic assembly
Bibliographic record
Abstract
We report the first demonstration of DNA oligonucleotide tags used to address the site-specific assembly of multiple redox enzymes onto spatially distinct regions of a nanoelectronic platform, establishing a direct electrical contact. The resulting system constitutes a multiplexed carbon nanotube-redox protein biosensor capable of detecting varying concentrations of several different substances in real time. The efficiency and robustness of the enzyme linking scheme is explored in detail, showing a high degree of preservation of enzymatic activity and an efficient electrical contact at the enzyme-nanoelectrode interface. While five proteins have been used as a demonstration in this study, there is virtually no limit to the number of enzymes that could be bound in parallel using this linking strategy, which is universally applicable to all proteins due to the simple conjugation chemistry involved. We further demonstrate metallization of the linker in the presence of a divalent metal cation, inducing elevated electron transfer efficiency relative to the native DNA link.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".