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Record W2133373890 · doi:10.5539/ijc.v4n6p75

Chemical Synthesis and NMR Characterization of Non Steroidal Mimics of an Estradiol Derivative Used as Inhibitor of 17beta-Hydroxysteroid Dehydrogenase Type 1

2012· article· en· W2133373890 on OpenAlexafffundvenue
Guy Bertrand Djigoué, René Maltais, Charles Ouellet, Alexandre Trottier, Donald Poirier

Bibliographic record

VenueInternational Journal of Chemistry · 2012
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicEstrogen and related hormone effects
Canadian institutionsCentre hospitalier de l'Université Laval
FundersCanadian Institutes of Health Research
KeywordsChemistryStereochemistryDehydrogenaseHydrobromideAmino acidEnzymeHydrolysisIn vitroNucleophilic substitutionNucleophileProtecting groupCombinatorial chemistryCatalysisBiochemistryAlkylOrganic chemistry

Abstract

fetched live from OpenAlex

Inhibiting estradiol (E2) biosynthesis through 17?-hydroxysteroid dehydrogenase type 1 (17?-HSD1) inhibitors is a promising strategy for breast cancer therapy. We have designed a non-steroidal template to mimic CC-156, a potent steroidal inhibitor of 17?-HSD1. Starting from tetrahydro-isoquinolinol hydrobromide, two representative compounds were synthesized in six chemical steps: protection of the amino group, protection of the phenol, hydrolysis of the N-protecting group Fmoc, nucleophilic substitution to introduce an ethyloxirane, phenolysis with meta or para hydroxybenzamide and the hydrolysis of the MOM protecting group. Although the compounds showed a good fit when docked in the catalytic site of the enzyme, conserving the key interactions with amino acids His221 and Ser142, observed from the crystalline structure of inhibitor CC-156 with 17?-HSD1, they weakly inhibited 17?-HSD1. However, they did not show estrogenic activity when tested in vitro, suggesting the potential of this non-steroidal template for drug design. Furthermore, the synthetic approach reported here opens a door to the preparation of additional non-steroidal mimics of E2 derivatives, which could be tested on 17?-HSD1 and others biological targets.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.450

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.006
GPT teacher head0.246
Teacher spread0.240 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations2
Published2012
Admission routes3
Has abstractyes

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