Expression of GroEL and DnaK Proteins during the Acquisition of a Transitory Resistance to Lethal Stresses by<i>Actinobacillus actinomycetemcomitans</i>
Bibliographic record
Abstract
Actinobacillus actinomycetemcomitans is a periodontopathogenic bacterium associated with active lesions of localized aggressive periodontitis. Cells of A. actinomycetemcomitans grown to mid-log phase, diluted or undiluted, and subjected to a sublethal heat stress demonstrated a partial protection to a subsequent lethal heat stress. On the contrary, stationary phase heat-stressed cells did not exhibit this transient thermal protection. Heat-stressed cells at sublethal temperature showed a higher expression of GroEL and DnaK proteins by immunoblotting, which was in agreement with de novo synthesis. A. actinomycetemcomitans was found to be more sensitive to a pH than to a heat stress. A transient protection to a lethal acid stress was demonstrated when cells were previously subjected to a sublethal acid stress. On the contrary, such transient protection did not occur for alkaline stresses. DnaK but not GroEL proteins made up a high proportion of the total protein synthesized following acid stress. The protection phenomenon is highly complex and heat shock proteins, which may help A. actinomycetemcomitans to support subsequent lethal conditions, are not expressed in the same way during the different stresses investigated. Key words: Actinobacillus actinomycetemcomitans , periodontitis, heat shock protein, stress tolerance.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".