The acetal levulinyl ester (ALE) group for the 2'-hydroxyl protection of ribonucleosides and the synthesis of oligoribonucleotides
Bibliographic record
Abstract
A novel 2'-O-ALE (Acetal Levulinyl Ester) uridine phosphoramidite derivative has been efficiently prepared and used for the solid-phase synthesis of a chimeric oligonucleotide strand, 5'-rU11-dT-3'. The average coupling yield of the phosphoramidite was comparable to one obtained with a 2'-TBDMS rU phosphoramidite reagent. Upon completion of the RNA chain assembly, the cyanoethyl phosphate protecting groups were removed using a solution of triethylamine in acetonitrile (2:3 v/v). The 2'-O-ALE protecting groups were cleaved under hydrazinolysis conditions. Finally, the RNA oligonucleotide was released from the Q-CPG support when treated with 1 molar TBAF in THF. When the TBAF step was carried out prior to hydrazinolysis, an oligonucleotide with its intact 2'-O-ALE groups was obtained.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.002 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".