Key Role of Promoter Methylation and Inactivation of MCPH1 Gene in Brain Tumors
Bibliographic record
Abstract
Background: Microcephalin (MCPH1/BIRT1) is a putative tumor suppressor gene which has recently seized great attentions in cancer studies. The present study was conducted to seek the impact of MCPH1 promoter methylation on development of brain tumors and telomere repeat length (TRL). Methods: The brain tissue section provided from brain tumor patients and two normal brain autopsies were undergone DNA isolation. DNA samples treated with bisulfite sodium using DNA modification kit (Qiagen) were amplified in methylation specific polymerization chain reaction (MSP-PCR) confirmed by sequencing. Protein expression analysis was performed by immunofluorescence (IF) assay using antibodies against MCPH1, cyclin E and CDC25A proteins. The TRL of brain tumor patients was determined through quantitative fluorescent in situ hybridization (Q-FISH). Results: The MCPH1 gene promoter was methylated in 96.6% of the patients consistent with protein expression pattern and the telomere statue was confirmed by low or absence of signal in tumor cells. Conclusion: MCPH1 promoter methylation had strong association with TRL, tumor’s grade and stage (P < 0.05). TRL was meaningfully associated with grade and subtype pathology of brain tumors (P = 0.01). Further studies are required to clarify the exact role of MCPH1 gene on TRL and tumor suppression especially in brain tumors. J Neurol Res. 2014;4(5-6):133-138 doi: http://dx.doi.org/10.14740/jnr295e
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".