Populations related to <i>Alkanindiges</i> , a novel genus containing obligate alkane degraders, are implicated in biological foaming in activated sludge systems
Bibliographic record
Abstract
Activated sludge mixed liquor and biological foam samples were collected from five full-scale municipal wastewater treatment plants in Illinois, all of which were exhibiting biological foaming at the time of sampling. Oligonucleotide probe hybridization consistently measured higher levels of Gammaproteobacteria rRNA in the foam as compared with the mixed liquor for all treatment plants analysed. Cloning and sequencing of 16S rRNA gene amplicons led to the identification of populations which were abundant in each of the treatment plants. These populations were related to the Alkanindiges/Acinetobacter cluster within the Gammaproteobacteria. Further analysis of the 16S rRNA sequences indicated that they clustered in three phylogenetic groups outside the main Alkanindiges/Acinetobacter cluster, suggesting that these groups may represent new taxa. Terminal-restriction fragment length polymorphism analysis showed that these populations were enriched in the foam compared with the underlying mixed liquor similar to the enrichment of the Gammaproteobacteria measured by oligonucleotide probe membrane hybridization. The observed enrichment in foam samples is suggestive of a role for these populations in foam formation or stabilization, and their presence in all treatment plants analysed in this study may be indicative of their widespread abundance in foaming plants.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".