Partial Characterization of Protease from the Leaves of Jatropha curcas
Bibliographic record
Abstract
The crucial roles of proteases in food and other industries stimulate research to find additional sources of the enzyme especially from non-conventional sources. In this study, protease was partially characterized from Jatropha curcas leaves.The enzyme had pH and temperature optima of 4.0 and 45oC respectively. A decline in residual activity of the enzyme was observed at above 45oC and the activation energy (Ea) from Arrhenius plot was 0.57kJ/mol. The enzyme showed specificity in the order; casein>hemoglobin> albumin> ovalbumin. Initial velocity studies for the determination of kinetic parameters revealed a KM and Vmax of 0.48 mg/ml and 0.014µmol/min respectively with a computed index of physiological efficiency (Kcat) of 0.029 min-1. Furthermore, Dixon-Webb’s plot identified ionizable groups at the active site with pKa1 and pKa2 of 5.0 and 5.3 respectively as well as an enthalpy of ionization of 0.047 kcal/mol implicating aspartate as an important amino acid at the active site of the enzyme. The protease was highly sensitive to cysteine protease specific inhibitor iodoacetate while 1,10phenanthroline and ethylenediaminetetraacetate slightly inhibited the enzyme. Data from this study suggest that J. curcas protease possesses closely similar properties to other known industrial proteases.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".