Compatible Pollinations in Solanum chacoense Decrease Both S-RNase and S-RNase mRNA
Bibliographic record
Abstract
Gametophytic self-incompatibility (GSI) allows plants to block fertilization by haploid pollen whose S-allele constitution matches one of the two S-alleles in the diploid styles. GSI in Solanum chacoense requires a stylar S-RNase, first secreted from cells of the transmitting tract then imported into incompatible (self) pollen tubes. However, the molecular mechanisms allowing compatible pollen to evade S-RNase attack are less clear, as compatible pollen tubes also import S-RNase. Using styles of the same age and size in order to lower the degree of inter-style variability in S-RNase levels, we observe reduction of up to 30% of the total non-self stylar S-RNase in vivo during compatible crosses, whereas no degradation of self S-RNases is detected. This marked difference in stylar S-RNase levels dovetails with measurements of pollen-specific Lat52 mRNA, which decreases four-fold in incompatible compared to compatible crosses. Unexpectedly, we also find evidence for a reciprocal signaling mechanism from compatible pollen to the cells of the transmitting tract that results in a roughly three-fold decrease in S-RNase transcript levels. These findings reveal a previously unsuspected feedback loop that may help reinforce the compatible reaction.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".