Quantitative comparison of sarcomeric phosphoproteomes of neonatal and adult rat hearts
Bibliographic record
Abstract
Neonatal hearts respond to stress and function in an environment quite different from adult hearts. There is evidence that these functional differences not only reflect modifications in the abundance and isoforms of sarcomeric proteins but also in the modulation of sarcomeric protein phosphorylation. Yet our understanding of changes in sarcomeric protein phosphorylation in development is incomplete. In the experiments reported here, we first quantified the intact sarcomeric protein phosphorylation status between neonatal and adult rat hearts by employing comparative two-dimensional (2-D) gel electrophoresis in conjunction with phosphoprotein-specific staining. Subsequently, we measured phosphorylation changes at the peptide level by employing high-resolution linear ion trap-Fourier transform (LTQ-FT) mass spectrometry analysis of titanium dioxide-enriched phosphopeptides differentially labeled with (16)O/(18)O during in-gel digestion. We also employed Western blot analysis using phosphorylation site-specific antibodies to measure phosphorylation changes. Our data demonstrated the novel finding that phosphorylation levels of myosin-binding protein C (MyBP-C) at Ser(295) and Ser(315) as well as tropomyosin at Ser(283) increased, whereas phosphorylation levels of MyBP-C at Ser(320) and myosin light chain 2 at Ser(15) decreased in neonatal hearts compared with the same sites in adult hearts. Although our data highlight the significant challenges in understanding relations between protein phosphorylation and cardiac function, they do support the hypothesis that developmental changes in the modulation of protein are functionally significant and correlate with the prevailing physiological state.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.002 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".