The muscle-specific microRNAs <i>miR-1</i> and <i>miR-133</i> produce opposing effects on apoptosis by targeting HSP60, HSP70 and caspase-9 in cardiomyocytes
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Post-publication record
- Nature
- Retraction
- Reason
- Breach of Policy by Author;Duplication of/in Image;
- Date
- 9/13/2011 0:00
- Flagged by OpenAlex?
- Yes
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Abstract
The microRNAs miR-1 and miR-133 are preferentially expressed in cardiac and skeletal muscles and have been shown to regulate differentiation and proliferation of these cells. We report here a novel aspect of cellular function of miR-1 and miR-133 regulation of cardiomyocyte apoptosis. miR-1 and miR-133 produced opposing effects on apoptosis, induced by oxidative stress in H9c2 rat ventricular cells, with miR-1 being pro-apoptotic and miR-133 being anti-apoptotic. miR-1 level was significantly increased in response to oxidative stress. We identified single target sites for miR-1 only, in the 3'-untranslated regions of the HSP60 and HSP70 genes, and multiple putative target sites for miR-133 throughout the sequence of the caspase-9 gene. miR-1 reduced the levels of HSP60 and HSP70 proteins without changing their transcript levels, whereas miR-133 did not affect HSP60 and HSP70 expression at all. By contrast, miR-133 repressed caspase-9 expression at both the protein and mRNA levels. The post-transcriptional repression of HSP60 and HSP70 and caspase-9 was further confirmed by luciferase reporter experiments. Our results indicate that miR-1 and miR-133 are involved in regulating cell fate with increased miR-1 and/or decreased miR-133 levels favoring apoptosis and decreased miR-1 and/or miR-133 levels favoring survival. Post-transcriptional repression of HSP60 and HSP70 by miR-1 and of caspase-9 by miR-133 contributes significantly to their opposing actions.
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The record
- Venue
- Journal of Cell Science
- Topic
- MicroRNA in disease regulation
- Field
- Biochemistry, Genetics and Molecular Biology
- Canadian institutions
- Université de MontréalMontreal Heart Institute
- Funders
- —
- Keywords
- BiologyHSP60microRNAApoptosisPsychological repressionHsp70Cell biologyCaspase 3Molecular biologyCaspase 2Regulation of gene expressionHeat shock proteinGene expressionGeneGeneticsProgrammed cell death
- Has abstract in OpenAlex
- yes