Expressed Sequence Tag Analysis of Lilium longiflorum Generative Cells
Why this work is in the frame
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Bibliographic record
Abstract
The generative cell, the male gametic cell progenitor in flowering plants, undergoes mitotic division to produce two sperm cells. We have examined the gene expression profile of the Lilium longiflorum (lily) generative cell by sequencing expressed sequence tags (ESTs). A total of 886 ESTs derived from the generative cell cDNA library were clustered into 637 unique ESTs comprising 123 cluster ESTs and 514 singleton ESTs. Thirty-nine percent of non-redundant ESTs showing similarity to Arabidopsis genes with known function were thus assigned putative functions. Genes related to the ubiquitin system were abundant, suggesting the key role of ubiquitin-dependent proteolysis in gametogenesis. A total of 168 and 129 non-redundant lily generative cell ESTs showed significant similarity to maize sperm cell ESTs and Arabidopsis male gametophyte-specific transcripts, respectively. Fifty-five ESTs appeared to have significant similarities to both maize sperm cell ESTs and Arabidopsis male gametophyte-specific genes, indicating conservation of male gamete-expressed genes across different plant genera. Thus our data provide a handle to identify Arabidopsis gamete-expressed genes and to investigate their function. Several of these genes are potential candidates for analyzing the molecular basis of fertilization and for investigating mechanisms of gamete-specific transcriptional regulation in Arabidopsis through bioinformatics-based approaches.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it