Discrimination between UV radiation‐induced and thermally induced spectral changes in AT‐paired DNA oligomers using UV resonance Raman spectroscopy
Bibliographic record
Abstract
Abstract Ultraviolet resonance Raman spectroscopy (UVRRS) was used to monitor UV radiation and thermal energy deposition in 12‐ and 18‐mer AT oligomers. Difference spectroscopy and two‐dimensional correlation spectroscopy (2DCOS) were used to characterize the distinct spectral responses manifested by the two processes, and to examine their potential cooperative and/or independent effects on the ensemble spectrum. Experiments utilizing incremental doses of 257‐nm radiation revealed that the most affected bands are those involving double bonds when the sample temperature was held constant. Complementary experiments utilizing sample heating indicated that the most affected bands were those involving hydrogen‐bond disruption. Finally, it is shown that bands associated with Watson–Crick hydrogen‐bond disruption are the most affected in rapidly heat‐cycled samples. The results also suggest that both radiation and thermal effects produce independent structural changes in AT oligomers and, furthermore, that these effects are complementary when the experiments involve single‐strand DNA fragments. When using UVRRS to study one of these perturbations (e.g. thermal stability of DNA), the concurrent perturbation (e.g. UV exposure) must not be neglected, since UV exposure is inherent in the UVRR process. These findings thus have implications for the use of UVRRS in the study of DNA dynamics. Copyright © 2006 John Wiley & Sons, Ltd.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".