Pressurized liquid extraction of toxins from cyanobacterial cells
Why this work is in the frame
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Bibliographic record
Abstract
The suitability of pressurized liquid extraction (PLE) of cyanotoxins from cells was investigated. The stability of cyanotoxins (MCYST-RR, MCYST-LR, and anatoxin-a) was evaluated at nine combinations of pressure and temperature (7, 10, and 14 MPa and 60 degrees C, 80 degrees C and 100 degrees C) using 75% (v/v) methanol in water (MeOH) as solvent. Additional experiments investigated the stability of cyanotoxins when water was used as solvent (at a pressure of 14 MPa and a temperature of 40 degrees C, 50 degrees C, 60 degrees C, 80 degrees C, or 100 degrees C). Results using 75% MeOH showed that the MCYST-RR and MCYST-LR were stable under the tested pressures up to 80 degrees C. At 100 degrees C MCYST recovery decreased by 10% to 17%. When water was used as the solvent, no differences in recovery were observed for MCYST-LR, whereas for MCYST-RR, maximum recovery was obtained at 60 degrees C, and degradation occurred at 100 degrees C. In contrast, anatoxin-a was labile under all experimental conditions; the best recoveries (ca. 50%) were obtained at 60 degrees C at the three pressures using 75% MeOH. However, only 17%-23% recovery was obtained with water extraction at all temperatures. The extraction of MCYST-LR and variants from cells (Microcystis aeruginosa, UTCC299) was studied using two solvents, 75% MeOH and 100% water, at 14 MPa and 60 degrees C and 100 degrees C. PLE extracts were compared with extracts obtained with 75% MeOH and ultrasonication. Complete extraction was achieved in both solvents in one 5-min cycle (at 100 degrees C). Although lower recovery was obtained using PLE (79%-105%), shorter extraction time and automation are advantageous over ultrasonication.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.027 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it